Table 1.
Locus | Primer sequence (5’-3’) | EAS (bp) | Ta/°C |
---|---|---|---|
MAA54a |
F: AACCTCCAAACACTAGGACAAC R: AGCTACTTCCAAAACGTGACA |
211–239 | 59 |
MAA105 |
F: GTTGGGACACTGTGAACTATTC R: AAAAGCTAGTGGATTAGATGCA |
265–293 | 60 |
MAA122 |
F: CTGGCCTTCAGTTTTGTCAAC R: CACCAGGCTTCAAGATGAAA |
254–312 | 55 |
MAA140 |
F: CCATCCCCCATCTTTCCT R: TCCTCGTTTGCCACAGTG |
142–160 | 55 |
MAA145 |
F: CCAACGCATAGCCAAATC R: AAATCCCAAACCCAACGT |
275–299 | 50 |
MAA156 |
F: CTGGTGCTTCAGCCTAATG R: TCAGCGTCAAAGATAACTCG |
283–307 | 55 |
MAA178a |
F: GATGGAGACACTTTGAACTAGC R: CACCAGGGTTTAAGATGAAAC |
250–258 | 55 |
MAA182 |
F: TACTGGGTCTGAAAAGATGTCT R: CGTTTGCGTTTGGATAAAT |
186–216 | 54 |
MAA196a |
F: GGAATGTGGTAGATGAAACTCC R: GACAAAAAAACAAAGGAAGAC |
283–315 | 57 |
MAA196b |
F: GAATGTGAGAGATAAATCTCC R: GACAAAAAAACAAAGGAAGAC |
337–377 | 46 |
EAS = expected allele size, Ta = annealing temperature