Fig. 2.

tal1∆ and pfk2∆ enhance shinorine production by independent mechanisms in yeast. (A) A schematic showing the glycolytic pathway, the pentose phosphate pathway and other reactions related to sedoheptulose 7-phosphate metabolism in S. cerevisiae. Blocking the phosphofructokinase reaction would theoretically increase flux through the oxidative branch of the PPP via ZWF1 (as indicated in red). (B) Comparison of shinorine levels in the wild-type and pfk2∆ strains. (C) The levels of fructose 6-phosphate produced in wild-type, tal1∆, pfk1∆, and pfk2∆ strains. (D) The effect of tal1∆, pfk1∆, pfk2∆, zwf1∆ and pfk1∆pfk2∆ on levels of S7P present in the cell, which correspond to the levels of shinorine produced. (E) Comparison of shinorine levels in the wild-type, pfk2∆, tal1∆ and pfk2∆tal1∆ strains. Error bars represent the standard deviation (SD) of three independent experiments. Significance was determined using an unpaired t-test. In Fig. 2D, the asterisks above the sample bars denote significance relative to the wild-type strain. ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001