Fig. 3.

The pfk2∆-mediated boost in MAA production is not due to increased flux via PPP. (A) Glucose 6-phosphate obtained from hexokinase-mediated phosphorylation of glucose can be metabolized either via the glycolytic pathway through PGI1, or via the pentose phosphate pathway through ZWF1. (B) Comparison of shinorine levels in the wild-type, zwf1∆, pfk2∆ and pfk2∆zwf1∆ strains. (C) Relative levels of glucose 6-phosphate in the wild-type, pfk1∆ and pfk2∆ cells. Error bars represent the standard deviation (SD) of three independent experiments. Significance was determined using an unpaired t-test. The asterisks above the sample bars denote significance relative to the wild-type strain. **** p < 0.0001. (D) Comparison of MAA levels in the negative control, wild-type, pfk2∆ and pfk2∆tkl1∆tkl2∆ strains