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. 2024;17(1):45–56. doi: 10.22037/ghfbb.v17i1.2779

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© 2024, Gastroenterology and Hepatology From Bed to Bench (GHFBB)

This is an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) which permits others to copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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Agarose gel electrophoresis results of Adenovectors constructing steps; (A) A 210 bp band indicated the insertion of Mda7 gene in transfer plasmid(B) A 126 bp band showed the presence of Hexon gene in main plasmid (C) Mini-preparation of recombinant Adenovector showed two bands with a significant distance to 10 kb ladder, revealing the presence of a big backbone construct, adenovector (D) Recombinant adenovector digestion by Pac1. The pattern showed 4.5 and >10 Kbp bands indicating the integrity of the new Ad-Mda7-tLyP1 construct