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. 2023 Aug 25;2(9):e97. doi: 10.1002/jex2.97

FIGURE 3.

FIGURE 3

Pretreatment with clodronate‐encapsulated liposomes does not improve EV‐mediated intracellular protein delivery. (a) Schematic illustration of study design comparing the effect of pretreatment with clodronate‐encapsulated liposomes of Cre‐EV i.v. injection. (b) Representative images from the live in vivo tracking by NIRF imaging of AlexaFluor790‐labeled Cre‐EVs in Ai9 mice that received (c) pretreatment with clodronate‐encapsulated liposomes or (d) only Cre‐EVs. (d) Immunohistochemistry‐ and (e) immunofluorescent images of liver sections from mice that received were pretreated with or without clodronate‐encapsulated liposomes. Red = tdTomato, blue = Hoechst, scale bar = 50 μm. (f) Immunofluorescence quantification of total tdTomato+Hoechst+ cells in liver tissue sections from Ai9 Cre‐loxP reporter mice that received either Cre‐EVs or pretreatment with clodronate encapsulated liposomes. Statistical analysis was performed with an unpaired t‐test, p‐values *<0.05, **<0.01, ***<0.001, and ****<0.0001 are considered statistically significant. Per group n = 2, total n = 4.