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. 2023 Aug 25;2(9):e97. doi: 10.1002/jex2.97

FIGURE 4.

FIGURE 4

Repeated injections of Cre‐EVs improved delivery to hepatocytes. (a) Schematic illustration of study design administrating a PBS (Vehicle), single dose‐ (D1), higher dose‐ (D2) or multiple doses (3xD1) or Cre‐EVs in Ai9 mice. (b) Immunofluorescence images from liver sections of Ai9 mice that received i.v. injection of the vehicle, D1, D2 or 3xD1. Red = tdTomato+, Blue = Hoechst. Scale bar = 50 μm. (c) Quantification of liver sections showed 3xD1 resulted in more tdTomato+Hoechst+ cells. (d) Immunofluorescence images of liver tissue section of Ai9 Cre‐loxP reporter mice from the 3xD1 group with antibodies against tdTomato+, HNF4A and Hoechst. Green = HNF4A, Red = tdTomato+, Blue = Hoechst, Scale bar = 100 μm. (e) Immunofluorescence images from different organs of Ai9 mice that received i.v. injection of the vehicle or 3xD1. Scale bar = 100 μm. Vehicle n = 3, D1 n = 3, D2 n = 2, 3xD1 n = 3, total n = 11.