FIGURE 4.

D‐BHI prepared BEVs have higher particle/protein ratios compared with those from standard BHI medium. (a) The particle numbers (pink) and protein concentrations (grey) of BEVs isolated from H. pylori bacteria grown in standard and D‐BHI media. (b) Particle/protein ratios as a measure of the relative purity of H. pylori BEVs prepared from the two media. Groups were compared by unpaired t‐test. ns = not significant. *p < 0.05, **p < 0.01, ****p < 0.0001. Error bars: Mean ± SEM. n = 4 biological replicates for both particle and protein concentration.