FIGURE 6.

BEVs isolated from D‐BHI induced similar IL‐8 production in AGS cells as those from BHI medium. AGS cells were stimulated with increasing doses of BEVs isolated from H. pylori grown in either BHI or D‐BHI. As negative controls, non‐stimulated (NS) cells and cells stimulated with 50 μg uninoculated samples of BHI, retentate fraction or D‐BHI were included. At 24 h post‐stimulation, supernatants were examined for IL‐8 levels by ELISA. Groups were compared using a one‐way ANOVA followed by Tukey multiple comparison test. ns = not significant. *p < 0.05, **p < 0.01, ***p < 0.001. Error bars: Mean ± SEM. n = 3 biological replicates.