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p300 can reverse the BSAP inhibition of PU.1 transactivation. (A) NIH 3T3 cells were transfected with the (PU.1/Pip)4LBKCAT reporter and the various expression plasmids indicated above each lane. BSAP represents clone BSAP Δ228–254. Lanes 4 to 6 contain 50 ng, 2.5 μg, and 5.0 μg of p300 expression plasmid, respectively. (B) Histogram of the p300 reversal of BSAP repression experiment in panel A. A duplicate experiment showed the same results.
