FIG. 10.

CaM inhibitors abolish the activation of ERKs induced by EGF in PC12 cells and by BDNF in chicken MTNs. (A) PC12 cells were pretreated or not pretreated (−) for 1 h with the indicated concentrations of the CaM inhibitor W13 and then stimulated (+) or not stimulated (−) for 5 min with EGF. (B) PC12 cells were pretreated (+) or not pretreated (−) with W13 for 1 h and then stimulated (+) or not stimulated (−) for the indicated times with EGF. (C) MTNs were pretreated (+) or not pretreated (−) for 1 h with W12 or W13 and then stimulated (+) or not stimulated (−) for 5 min with BDNF. ERK phosphorylation was determined as described in the legend to Fig. 1.