TABLE 1.
Oligos used in this study
| Name | Length (nucleotides) | Sequence (5′→3′) | Purpose |
|---|---|---|---|
| EST1-5Bam | 43 | GAATTCCCGGGGATCCGTATGGATAATGAAGAAGTTAACGAAG | PCR cloning of EST1 |
| EST1-3Pst | 39 | GGGCGTCGACCTGCAGTCAAGTAGGAGTATCTGGCACTT | PCR cloning of EST1 |
| N3HAEST1 | 36 | ACCCCAGATCTTTAAATACCCATACGATGTTCCTGA | 3HA tagging of EST1 |
| BglII3HA | 26 | CCCCAGATCTGAGCAGCGTAATCTGG | 3HA tagging of EST1 |
| EST1-RNP1A | 63 | GAGAAGATATTATTTTCGGGGAATTTTCTTGCGCTAACGCTGCACTAACAGATTTTAATGACG | Making RNP1-NC |
| EST1-RNP1B | 63 | CGTCATTAAAATCTGTTAGTGCAGCGTTAGCGCAAGAAAATTCCCCGAAAATAATATCTTCTC | Making RNP1-NC |
| EST1-RNP1C | 62 | GAGAAGATATTATTTTCAAGGAATTCTCTTGCGTTAACTTTGCACTAACAGATTTTAATGAC | Making RNP1-C |
| EST1-RNP1D | 62 | GTCATTAAAATCTGTTAGTGCAAAGTTAACGCAAGAGAATTCCTTGAAAATAATATCTTCTC | Making RNP1-C |
| EST1-RNP2A | 47 | GCACTTCATTCGCCGGGTTGCTGAACGACGCGATAAATAGTCCACTG | Making RNP2-ga |
| EST1-RNP2B | 47 | CAGTGGACTATTTATCGCGTCGTTCAGCAACCCGGCGAATGAAGTGC | Making RNP2-ga |
| EST1-RNP2C | 52 | GGAAGTTTTGCACTTCATTCGCCGGATCCCTGAACGACTTGATAAATAGTCC | Making RNP2-gs |
| EST1-RNP2D | 52 | GGACTATTTATCAAGTCGTTCAGGGATCCGGCGAATGAAGTGCAAAACTTCC | Making RNP2-gs |
| EST1F511SA | 48 | CATTAACTTTGCACTAACAGATTCTAATGACGATTATGTGTATGATTC | Making F511S |
| EST1F511SB | 48 | GAATCATACACATAATCGTCATTAGAATCTGTTAGTGCAAAGTTAATG | Making F511S |
| EST1D513IA | 45 | CTTTGCACTAACAGATTTTAATATCGATTATGTGTATGATTCTCC | Making D513I |
| EST1D513IB | 45 | GGAGAATCATACACATAATCGATATTAAAATCTGTTAGTGCAAAG | Making D513I |
| EST2KOUP | 70 | ATGAAAATCTTATTCGAGTTCATTCAAGACAAGCTTGACATTGATCTACAGACCAGGGAACAAAAGCTGG | Making est2Δ |
| EST2KODW | 70 | CAGCATCATAAGCTGTCAGTATTTCATGTATTATTAGTACTAATTAACTATATGCTATAGGGCGAATTGG | Making est2Δ |
| EST2KODGUP | 19 | CCATAACTAACACGCCCTC | Diagnosis of est2Δ |
| EST2KODGDW | 21 | GGCTTATTACAAAGTTTGCGG | Diagnosis of est2Δ |
| EST3KOUP | 79 | GGGATAACAAGTAAACAATGCCGAAAGTAATTCTGGAGTCTCATTCAAAGCCAACAGACTCAGAGGGAACAAAAGCTGG | Making est3Δ |
| EST3KODW | 75 | GCCTGCAGAAGGTCATAAATATTTATATACAAATGGGAAAGTACTTAACGATCCGACTTCTATAGGGCGAATTGG | Making est3Δ |
| EST3KODGUP | 20 | GGTCAGAATGGGCGCTTGTC | Diagnosis of est3Δ |
| EST3KODGDW | 21 | CTCTAGAGGAGTACTTATCGG | Diagnosis of est3Δ |
| TELPG | 27 | GTGTGTGGGTGTGTGTGTGGGTGTGTG | Telomere probe |