TABLE 3.
Summary of yeast single-stranded telomeric DNA bindinga
| Est1 protein | Ratio of 27-mer to 62-mer
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Data from expt:
| ||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | Avg | |
| Est1 | 1.7 | 1.8 | 2.3 | 2.3 | 2.4 | 2.1 | 1.4 | 2.0 |
| 3HA-tagged Est1 | 4.4 | 5.1 | 5.4 | 4.8 | 2.7 | 4.4 | 2.2 | 4.1 |
| Est1-RNP1-NC | 1.6 | 1.6 | 2.0 | 1.2 | 1.6 | |||
| 3HA-tagged Est1-RNP1-NC | 2.9 | 4.9 | 4.4 | 1.7 | 3.5 | |||
Data from 11 independent pairs of experiments are presented as the ratio of 27-mer (telomeric oligo) to 62-mer (nontelomeric oligo). Seven of these pairs of experiments compared the single-stranded DNA-binding activity of 12CA5 immunoprecipitates from untagged and tagged wild-type Est1 strains. The average ratio for untagged Est1 was 2.0; the average ratio for tagged Est1 was 4.1. By ANOVA, this difference was found statistically significant at the 0.05 level. The other four pairs of experiments compared the single-stranded DNA-binding activity of 12CA5 immunoprecipitates from untagged and tagged Est1-RNP1-NC strains. Again, ANOVA showed that the ratios (1.6 versus 3.5) were significantly different. In addition to the experiments shown here, there were one pair of experiments with wild-type Est1 and one pair of experiments with Est1-RNP1-NC (both of these pairs were done on the same day); they were excluded from the analysis because of abnormally high ratios in all parts of the experiments, including controls.