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. 2024 May 9;20(5):e1011253. doi: 10.1371/journal.pgen.1011253

Fig 3. SV-lysosomes in lrk-1 and apb-3 mutants are dependent on UNC-104.

Fig 3

(A) SNG-1::GFP in the cell body, process, and synapses of PLM neurons show dependence on UNC-104 in lrk-1(km17) and apb-3(ok429) mutants and their doubles with unc-104(e1265tb120). Scale bar: 10 μm. (B) CTNS-1::mCherry in the cell body, process, and synapses of PLM neurons shows dependence on UNC-104 in lrk-1(km17) and apb-3(ok429) mutants and their doubles with unc-104(e1265tb120). Red arrows highlight CTNS-1 puncta. Scale bar: 10 μm. (C) Quantitation of fraction of total moving SNG-1-carrying vesicles co-transporting CTNS-1 in unc-104(e1265tb120), lrk-1(km17); unc-104, and apb-3(ok429); unc-104 from kymograph analysis of sequential dual color imaging done at 1.3 fps. #P-value ≤ 0.05 (Mann–Whitney Test, all comparisons to WT); ns: not significant; Number of animals (N) ≥ 18 per genotype; Number of vesicles (n) > 1200.