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. 2024 May 9;20(5):e1011253. doi: 10.1371/journal.pgen.1011253

Fig 4. Distribution of SV-lysosomal compartments depends on UNC-104.

Fig 4

(A) Quantitation of fraction of total moving SNG-1-carrying vesicles co-transporting CTNS-1 in syd-2 mutants and their doubles with lrk-1(km17) and apb-3(ok429), from kymograph analysis of dual color imaging. ok217 refers to the null allele of syd-2, syd-2(ok217), while ju37 refers to the syd-2(ju37) allele. #P-value ≤ 0.05 (Mann–Whitney Test, all comparisons to WT); ns: not significant; Number of animals (N) ≥ 18 per genotype; Number of vesicles (n) > 750. Values for lrk-1 and apb-3 single mutants are the same as those in Fig 2B. (B) Quantitation of fraction of total moving SNG-1-carrying vesicles co-transporting RAB-7 in syd-2(ok217) and its doubles with lrk-1(km17) and apb-3(ok429), from kymograph analysis of dual color sequential imaging at 1.3 fps. P-value > 0.05 (One-Way ANOVA with Tukey’s post-hoc test); ns: not significant; N ≥ 19 per genotype; n > 700. Values for lrk-1 and apb-3 single mutants are the same as those in Fig 2C. (C) CTNS-1::mCherry in the cell body, process, and synapses of PLM neurons of syd-2(ok217) mutant and its doubles with lrk-1(km17) and apb-3(ok429). Red arrows highlight some CTNS-1-carrying compartments, some fainter. Scale bar: 10 μm. (D) mScarlet::RAB-7 in the cell body, process, and synapses of PLM neurons of syd-2(ok217) mutant and its doubles with lrk-1(km17) and apb-3(ok429). Red arrows highlight some RAB-7-carrying compartments, some fainter. Scale bar: 10 μm. (E) LMP-1::mNeonGreen in the cell body, process, and synapse of PLM neurons of syd-2(ok217) mutant and its doubles with lrk-1(km17) and apb-3(ok429). Red arrows indicate LMP-1-carrying compartments. Scale bar: 10 μm. (F) Quantitation of fraction of total moving SNB-1-carrying vesicles co-transporting RAB-3 in WT and syd-2(ok217), from simultaneous dual color imaging at 3 frames per second (fps). # P-value ≤ 0.05 (2 tailed Student’s t test); N > 20. (G) Quantitation of fraction of total moving SNG-1-carrying vesicles co-transporting RAB-3 in WT and syd-2(ok217) from sequential dual color imaging at 1.3 fps. P-value > 0.05 (One-Way ANOVA with Tukey’s post-hoc test); ns: not significant; N > 15. (H) CTNS-1::mCherry in the cell body, process, and synapses of PLM neurons of syd-2(ju37) mutant and its doubles with lrk-1(km17) and apb-3(ok429). Red arrows highlight some CTNS-1-carrying compartments, some fainter. Scale bar: 10 μm. Imaged at 100×.