TABLE 1.

Activation of both MybER and MycER reverses growth arrest in IL-6-treated M1 cells

Expta	Treatment	% cells in G1c
		M1/MybER		M1/MycER
		24 h	48 h	24 h	48 h
1	IL-6	54.7	71.6	54.9	69.6
	4-OHT	41.3	33.4	38.8	40.1
	IL-6 + 4-OHT	44.8	42.4	49.3	52.8
	% Inhibitionb	73.8	76.4	34.8	56.9
2	IL-6	59.0	60.6	63.9	66.3
	4-OHT	35.7	31.6	34.4	41.8
	IL-6 + 4-OHT	34.1	40.1	51.9	49.0
	% Reversal	107.0	70.7	40.7	70.6

^aIn experiment 1, cells were treated with IL-6 and 4-OHT at the same time to see if induction of c-Myb- and/or c-Myc activity would prevent movement of cells into G₁. In experiment 2, cells were first pretreated with IL-6 to allow the cells to enter G₁; after 72 h, 4-OHT was added to determine if activation of MybER or MycER could reverse the percentage of cells in G₁. 

^b% inhibition (reversal) = (IL-6− [IL-6 + 4-OHT])/(IL-6 − 4-OHT) × 100, where IL-6 − 4-OHT defines the percentage of cells that can maximally move into G₁ after induction of growth arrest by IL-6 and IL-6 − [IL-6 − 4-OHT] defines the percentage of cells in IL-6 growth-arrested cell cultures, after activation of the fusion protein with 4-OHT, that have been inhibited from entering or reversed from G₁. 

^cAverage of three independent experiments.