FIGURE 3.

Purified EVA‐ACA1001 binds to CC chemokines. (a–d) Representative surface plasmon resonance (SPR) sensorgrams show the binding of immobilized EVA‐ACA1001 to five increasing concentrations of chemokines (31.25, 62.5, 125, 250, and 500 nM). (e) Binding affinities (left axis: pK _d = −log[K _d, M]; right axis: K _d, nM) of EVA‐ACA1001 for CC chemokines, measured by SPR. Data are presented as mean pK _d ± SEM from three independent experiments. $, K _d <0.1 nM; #, no measurable binding at 500 nM chemokine concentration.