FIGURE 5.

Truncation of EVA‐ACA1001 termini has minimal effects on binding to chemokines. N‐terminal (green) or C‐terminal (blue) truncation of EVA‐ACA1001 has only small effects on chemokine binding affinity or selectivity. Binding affinities (left axis: pK _d = −log[K _d, M]; right axis: K _d, nM) are presented as mean pK _d ± SEM from three independent experiments. $, K _d <0.1 nM; #, no measurable binding at 500 nM chemokine concentration; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared to full‐length EVA‐ACA1001 (one‐way ANOVA with Šídák correction for multiple comparisons for each chemokine; or a two‐tailed t‐test if only a single comparison was possible).