TABLE 1.
Binding affinities (K d in nM) of EVA‐ACA1001 to human chemokines measured by surface plasmon resonance using multiple‐cycle kinetics.
| Chemokine | pK d ± SEM (K d nM) |
|---|---|
| CCL1 | 7.71 ± 0.13 (21.1) |
| CCL2 | 8.19 ± 0.08 (6.5) |
| CCL3 | 8.99 ± 0.12(1.08) |
| CCL4 | 8.93 ± 0.07 (1.18) |
| CCL5 | 9.55 ± 0.03 (0.27) |
| CCL7 | a |
| CCL8 | 8.34 ± 0.10 (4.76) |
| CCL11 | 9.49 ± 0.20 (0.38) |
| CCL13 | 9.41 ± 0.05 (0.39) |
| CCL14 | a |
| CCL15 | 7.02 ± 0.15 (107) |
| CCL16 | 9.34 ± 0.19 (0.54) |
| CCL17 | 8.99 ± 0.01 (1.01) |
| CCL18 | a |
| CCL19 | 8.90 ± 0.10 (1.31) |
| CCL20 | b |
| CCL21 | 8.19 ± 0.03 (6.50) |
| CCL22 | 8.90 ± 0.09 (1.29) |
| CCL23 | 8.93 ± 0.02 (1.17) |
| CCL24 | 8.22 ± 0.26 (8.90) |
| CCL25 | b |
| CCL26 | 8.77 ± 0.04 (1.68) |
| CCL27 | 7.07 ± 0.02 (84.8) |
| CCL28 | b |
Note: Data are reported as mean ± SEM (n = 3); the corresponding K d values (in nM) are given in parentheses.
a
K d < 0.1 nM.
b
No measurable binding at 500 nM chemokine concentration.