TABLE 2.
Effect of MafK overexpression on the α1 globin promoter activities in K562 cellsa
| Reporter plasmid | HS-40 | Cotransfected MafK | Mean relative promoter activityb ± SD (n) |
|---|---|---|---|
| pHS-40-α590GH | Wild type | − | 100 (8) |
| pHS-40-α590GH | Wild type | + | 32 ± 1 (8) |
| pHS-40(3′-NA-II)-α590GH | 3′-NA(II) | − | 75 ± 12 (9) |
| pHS-40(3′-NA-II)-α590GH | 3′-NA(II) | + | 83 ± 10 (9) |
a
K562 cells were first transiently cotransfected with pHS40-α590GH plus 1, 5, 10, 20, and 30 μg of pRSV-MafK, which resulted in promoter activities, as percentages of the activity of pHS-40-α590GH without cotransfected pRSV-MafK of 74 ± 7, 43 ± 11, 33 ± 11, 35 ± 10, and 26 ± 2, respectively. Two independent experiments were performed in duplicate.
b
Effect of 30 μg of cotransfected pRSV-MafK on activities of the α1 globin promoter linked in cis to wild-type HS-40 or HS-40 with the 3′-NA(II) mutation. The promoter strengths are relative to that of pHS-40-α590GH without cotransfected pRSV-MafK.