FIG. 4.

The SWI-SNF complex stimulates Gal4-τ1-driven transcription from chromatin templates. (A) A diagram showing the nucleosomal 5S-G5E4 array template. (B) A schematic representation of the in vitro transcription assay indicating the order in which the reagents were added. (C) The nucleosomal array template was transcribed following activator binding in the presence or absence of WT SWI-SNF complex or ATPase-deficient SWI-SNF complex (K798A-Swi2) as indicated. The HIV-1 DNA template was used as an internal recovery control. The transcripts marked E4 are subjected to regulation by the added Gal4 and Gal4-τ1 proteins. (D) Transcription of naked DNA template in the presence or absence of Gal4 or Gal4-τ1 and WT or ATPase-deficient (K798A-Swi2) SWI-SNF complex. The transcription conditions were the same as described for panel C, except for the replacement of the nucleosome array template with naked DNA and the absence of competitor nucleosomes.