FIG. 5.

hbrm potentiates GR τ1 activity in mammalian C33 cells. (A) Transactivation activity of the τ1c after cotransfection with expression plasmids τ1c-GRDBD and hbrm or hbrm-NTP mutant. The activity of the luciferase reporter gene in each condition is expressed relative to the activity of the τ1c in the absence of cotransfected plasmid encoding hbrm. The graphs represent mean values obtained from three independent transfections. (B) Western blot with affinity-purified hbrm antibodies. Extracts were prepared from C33 cells transfected with 20 μg of pCMV, pCMV-hbrm, or pCMV-NTP mutant.