FIG. 8.

The expression of the SRC-1 interaction domain of p300 (residues 2057 to 2170) inhibits activation by TR-RXR but not activation by GAL4-VP16. (A) The injection of DNA and mRNAs encoding TR-RXR and GAL4-VP16 was as described in the legend to Fig. 2C. The mRNA encoding p300 (residues 2057 to 2170) was injected (2 ng/oocyte) 2 h before the other mRNAs. The levels of transcription were quantitated with a PhosphorImager. (B) Expression of p300 (residues 2057 to 2170) shown by Western analysis with M2 antibody, specific for the Flag tag. p300 (residues 2057 to 2170) has a Flag tag at the N terminus. The molecular mass markers are indicated.