Table 2.

Primers for cytokines, antioxidants enzymes, and household gene

Target gene	Primer sequence	Amplified segment Length	Annealing temperature	Accession number
β-actin	F: AGCAAGCAGGAGTACGATGAG R: TGTGTGGTGTGTGGTTGTTTTG	135 bp	58.5 °C 30 s	XM_003443127.5
IL-1β	F: T GCTGAGCACAGAATTCCAG R: GCTGTGGAGAAGAACCAAGC	172 bp	60 °C 30 s	XM_019365841.2
IL-10	F: CTGCTAGATCAGTCCGTCGAA R: GCAGAACCGTGTCCAGGTAA	94 bp	60 °C 30 s	XM_013269189.3
TNF-α	F: CCAGAAGCACTAAAGGCGAAGA R: CCTTGGCTTTGCTGCTGATC	82 bp	59.9 °C 30 s	AY428948.1
SOD	F: GGTGCCCTGGAGCCCTA R: ATGCGAAGTCTTCCACTGTC	377	56 °C 30 s	JF801727.1
CAT	F: TCCTGAATGAGGAGGAGCGA R: ATCTTAGATGAGGCGGTGATG	232	56 °C 30 s	JF801726.1
GPx	F: CCAAGAGAACTGCAAGAACGA R: CAGGACACGTCATTCCTACAC	237	58 °C 30 s	NM_001279711.1

Note interleukin (IL), tumor necrosis factor (TNF-α), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)