Fig. 6. ITLN1 rs4656959 is associated with lower mucus plug scores in T2-high asthmatics.

a Box plots of log₂-normalized ITLN1 expression from 249 participants in the SARP asthma cohort stratified by T2 status and ITLN1 rs4656959 genotype; n: T2-low participants n: A/A = 43, A/G = 61, G/G = 13 and T2-high participants n: A/A = 37, A/G = 77, G/G = 18. Two-sided p values for indicated differences were obtained from a Wald test in DESeq2. Box centers = median, upper and lower box bounds = 1st and 3rd quartiles, whiskers extend from these bounds up to 1.5 × IQR (inter-quartile range). All data points are overlain. b Box plots of mucus plug scores (2 measurements per participant) from SARP, stratified into T2-low (n = 41 participants) and T2-high (n = 71 participants) groups based on sputum RNA-seq expression profiles. Two-sided p values were obtained by fitting negative binomial mixed model implemented in SAS PROC GLIMMIX (METHOD = RSPL; DDFM = KENWARDROGER2) with subjectID as random effect. Box plots as defined in a. c Box plots of mucus plug scores (2 measurements per participant) from SARP, stratified by both T2 status and ITLN1 rs4656959 variant genotype; T2-low participants n: A/A = 13, A/G = 26, G/G = 2 and T2-high participants n: A/A = 18, A/G = 43, G/G = 10. Two-sided p values were obtained by fitting a negative binomial mixed model implemented in SAS PROC GLIMMIX (METHOD = RSPL; DDFM = KENWARDROGER2) with subjectID as random effect. Box plots as defined in a. Source data are provided as a Source Data file.