FIG. 5.

Quantification of Met and Met activity in melanoma cells. Extracts prepared from 37-32 melanoma cells treated with either nothing (control), HGF/SF, or NK2 were immunoprecipitated (IP) with anti-Met antibody and subsequently probed with either anti-phosphotyrosine (α-pY, top left) or anti-Met (α-c-Met, bottom left) antibodies. Extracts were also directly probed with anti-phospho-MAPK antibody (right). Molecular masses in kilodaltons are shown. Note that, relative to NK2, HGF/SF induces phosphorylation of Met and MAPK without altering the levels of Met.