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. 2024 May 9;14:10678. doi: 10.1038/s41598-024-60257-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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LRBA immunofluorescence in salivary glands. Double-immunofluorescence confocal microscopy on mouse submandibular and parotid gland for LRBA (red) and for Na⁺/K⁺-ATPase (green) as a marker of basolateral plasma membranes. Nuclei were stained with DAPI. (c,f) Represent higher magnification images from the white-boxed areas in (b,e), respectively. LRBA immunofluorescence concentrated toward the subapical and apical cytoplasm of acinar and duct cells, and was abolished in KO control tissue (Supplementary Fig. S1b). A acinar cells, GD granular duct, ILD intralobular duct, SD striated duct. All scale bars: 25 µm.