Figure 5.

LRBA immunofluorescence in intestinal epithelia. Single LRBA immunofluorescence (red; a1,b1,c,c1,e1) and double immunofluorescence with Na⁺/K⁺-ATPase (green; a,a2,b,b2,e,e2), a marker for basolateral plasma membranes, and subsequent confocal microscopy on mouse intestine. Nuclei were stained with DAPI. (a1,a2,b1,b2,c1,e1,e2) Represent higher magnification images from the white-boxed areas in (a–c,e). In jejunum and colon, LRBA immunofluorescence concentrated at the apical cell poles and subapical regions of epithelial cells. In LRBA-KO tissue, LRBA IF was absent (d). V intestinal villi, L lumen. All scale bars: 25 µm.