Figure 4.

H4K12 and H3K14 ofT. gondiiare identified as histone lactylation sites

A. Western blotting of histones H4K12la and H3K14la. B. Indirect IFA of histones H4K12la and H3K14la in the intracellular and extracellular stages of the parasite. Rabbit IgG was used as the negative control. C. and D. MS/MS spectra of histone H4K12la (C) and H3K14la (D) obtained from T. gondii. The a and b ions refer to the N-terminal part of the peptide, and the y ion refers to the C-terminal part of the peptide. E. Distribution of the binding regions of histone lactylation sites (H4K12la and H3K14la) on the chromosome shown in circos map. The outermost circle shows the chromosomes and the inner circles show the distribution trend for each sample. Data represent three independent experiments. F. Distribution of overlapping H4K12la and H3K14la peaks in the whole genome. The length of the circled bars represents the degree of enrichment and the position within the circle corresponds to the position of the peaks. ChIP-seq, chromatin immunoprecipitation sequencing.