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. 2022 Oct 7;21(6):1163–1181. doi: 10.1016/j.gpb.2022.09.010

Figure 6.

Figure 6

Preliminary analysis of the function of TgHDAC2, TgHDAC3, TgHDAC4, and TgMYST-A in associated with protein lactylation

A. Sequence characteristics of TgHDAC2, TgHDAC3, TgHDAC4, and TgMYST-A. B. Western blotting analysis of the expression of native TgHDAC2 (71 kDa), TgHDAC3 (53 kDa), TgHDAC4 (101 kDa), and TgMYST-A (56 kDa) with protein-specific antibodies. C. Indirect IFA (co-localization) of TgHDAC2, TgHDAC3, TgHDAC4, and TgMYST-A. D. Flow chart on the effect of specific antibodies on PTMs. E. Modulation of anti-TgHDAC2, anti-TgHDAC3, anti-TgHDAC4, and anti-TgMYST-A antibodies on lactylation, crotonylation, 2-hydroxyisobutyrylation, and acetylation of T. gondii. Proteins with variation in modification level was pointed with an arrow. β-actin was used for normalization. HDAC2, HADC3, and HDAC4 are histone deacetylases; MYST-A is a kind of histone lysine acetyltransferase.