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. 2024 May 9;221(7):e20221122. doi: 10.1084/jem.20221122

Table 3.

gRNA sequences, related to experimental procedures

Cleavage site Guide ID Direction Sequences (5′-3′) PAM
STX11 intron 1 i1 Forward 5′-TGC​ACT​TAT​TGC​CCA​CACCG-3′ AGG
STX11 exon 2 e1 Forward 5′-TGA​CCA​GCA​GTT​CCC​AGACG-3′ GGG
STX11 exon 2 e2 Reverse 5′-TCG​AAC​ACG​ATG​TCC​TC GTG-3′ GGG
STX11 exon 2 e3 Reverse 5′-CTT​TCC​CAG​CCG​CTT​CACGT-3′ CGG
STX11 exon 2 e4 Forward 5′-AGT​CTC​GGG​CGA​CCA​GATCG-3′ AGG
STX11 exon 2 e5 Reverse 5′-GTT​CTT​CTC​CTC​GTA​CTGCA-3′ CGG