FIG. 1.

Isolation by ligand screening of cDNA clones that encode DNA-binding factors. Phages derived from an HL60 λgt11 expression library were induced to express fusion proteins and incubated with CpG-pos (20) or CGD oligonucleotide probe (40) as described in Materials and Methods. Shown are a representative phage clone that encodes a non-sequence-specific DNA-binding activity (top) and a representative phage clone that exhibits sequence-specific DNA-binding activity (bottom).