Table 1.

List of microfluidic qPCR assays used to simultaneously quantify multiple taxonomic and functional groups involved in sulfate reduction, as well as total prokaryotes.

Assay #	Taxonomic/functional group	Gene	Primer	Sequence (5′ −3′)	Reference
1	Desulfovibrio	16S rRNA	DSV691F	CCGTAGATATCTGGAGGAACATCAG	[24]
			DSV826R	ACATCTAGCATCCATCGTTTACAGC
2	Bilophila	tpa	tpa-FP	CGCCGGTATCGAAATCGTGA	[25]
			tpa-RP	ATTCGCGGAAGGAGCGAGAG
3	Sulfate reducers	dsrA #1a	Dsr1F+	ACSCACTGGAAGCACGGCGG	[26]
			Dsr1R	GTGGMRCCGTGCAKRTTGG
4	Sulfate reducers	dsrA #2a	RH1-dsr-F	GCCGTTACTGTGACCAGCC	[27]
			RH3-dsr-R	GGTGGAGCCGTGCATGTT
5	Sulfate reducers	dsrA #3a	dsrA-F	CCAACATGCACGGYTCCA	[28]
			dsrA-R	CGTCGAACTTGAACTTGAACTTGTAGG
6	Total prokaryotes	16S rRNA	515F	GTGYCAGCMGCCGCGGTAA	[29,30]
			806R	GGACTACNVGGGTWTCTAAT

^aDifferent numbers indicate different primer sets targeting the same gene.