| Reagent | Final concentration | Quantity or Volume |
|---|---|---|
| Tris base | 200 mM | 2.42 g |
| Potassium acetate | 500 mM | 4.91 g |
| Magnesium acetate | 150 mM | 2.14 g |
| Acetic acid | n/a | see note* |
| Total | n/a | 100 mL |
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