Reagent	Final concentration	Quantity or Volume
EDTA (0.5 M, pH 8.0) SDS Bromophenol blue Xylene cyanol Formamide	5 mM 0.025% 0.01% 0.005% 95%	10 mL 0.025 g 0.01 g 0.005 g see note*
Total	n/a	100 mL