TABLE 2.

H1 subtype composition of testis chromatin from wild-type and H1t^−/− mice^a

Genotype	% of indicated subtype						Total H1/nucleosome ratiod
	H1o	H1b	H1a	H1d + H1e	H1c	H1t
Total H1b
+/+	1.4 ± 0.7	1.2 ± 0.7	38.6 ± 2.4	10.8 ± 1.1	20.1 ± 1.6	27.7 ± 2.3	0.67 ± 0.08
−/−	2.2 ± 0.3	0.4 ± 0.1	51.2 ± 4.2	14.0 ± 2.2	31.8 ± 2.9	0	0.68 ± 0.08
H1a–H1e, H1oc
+/+	2.0 ± 0.9	1.5 ± 0.9	53.4 ± 2.1	15.0 ± 1.8	28.2 ± 1.3
−/−	2.2 ± 0.3	0.4 ± 0.1	51.2 ± 4.2	14.0 ± 2.2	31.8 ± 2.9

^aData were from HPLC analyses like that in Fig. 2. Values are means ± standard deviations of individual determinations made for three 3-month-old littermates of each of the indicated genotypes. 

^bDetermined by the ratio of the A₂₁₄ of the indicated H1 peak to the total A₂₁₄ of all H1 peaks. The percentages were adjusted to account for the differences in the number of peptide bonds in each H1 subtype. 

^cDetermined by the ratio of the A₂₁₄ of the indicated H1 peak to the total A₂₁₄ of the H1a to H1e and H1^o peaks. 

^dDetermined by the ratio of the total A₂₁₄ of all H1 peaks to half of the total A₂₁₄ of the H2b peaks. The A₂₁₄ values of the H1 and H2b peaks were adjusted to account for the differences in the number of peptide bonds in each H1 subtype and H2b.