Figure 1.

In vitro assays. (A) Dose-response curves for AGS R-5FU and AGS WT. mRNA expression levels of (B) TS and (C) CCR3 in AGS R-5FU and AGS WT. (D) Cell viability of AGS R-5FU cells treated with 5-FU and/or CCR3 antagonist, SB 328437, for 72 h. mRNA expression levels of (E) CCR3 and (F) TS after treatment with SB 328437 and/or 5-FU. Data were expressed as mean ± standard deviation of three biological replicates. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. AGS WT, wild type AGS cells; 5FU, 5-fluorouracil; AGS R-5FU, 5-FU resistant AGS cells; TS, thymidylate synthase; CCR3, C-C motif chemokine receptor 3; ns, not significant.