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. Author manuscript; available in PMC: 2024 May 10.
Published in final edited form as: Am J Transplant. 2023 Oct 31;24(4):564–576. doi: 10.1016/j.ajt.2023.10.026

Figure 8.

Figure 8.

5-ethynyl-2’-deoxyuridine (EdU) proliferation assay images and the number of EdU+ cells after 6 hours of jejunal and ileal CS or NMP. (A) EdU (purple) whole mount immunofluorescence on jejunal and ileal T0, CS-T6, and NMP-T6 spheroids. (B) NMP-T6 jejunal and ileal spheroids contained a great number of EdU+ cells compared with T0 or CS-T6. T0 = 10 pigs, CS-T6 = 7 pigs, and NMP-T6 = 8 pigs. Original magnification: 20×, scale bar: 50 μm. *P ≤.05, **P ≤.01, ***P ≤.001, and ****P ≤.0001. CS, cold storage; CS-T6, 6 hours of cold storage; NMP, normothermic machine perfusion; NMP-T6, 6 hours of normothermic machine perfusion; T0, control tissue.