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EdU proliferation assay. EdU proliferation assay was used to determine the number of EdU+ cells (mean ± standard deviation) in spheroids cultured from T0, CS-T6, and NMP-T6 ileal crypts.
| Ileal EdU proliferation assay | |||
|---|---|---|---|
| Number of EdU+ cells (mean ± standard deviation) | |||
| Cell Culture Duration | T0 | CS-T6 | NMP-T6 |
| 48 h | 24.88 ± 21.32 | 10.81 ± 10.25 | 39.58 ± 27.19 |
CS-T6, 6 hours of cold storage; EdU, 5-ethynyl-2’-deoxyuridine; NMP-T6, 6 hours of normothermic machine perfusion; T0, control tissue.
