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. 2024 Apr 30;124(9):6051–6077. doi: 10.1021/acs.chemrev.4c00004

Table 3. Symmetrical Bis-Electrophiles for Cyclization of Two-Cysteine Phage Libraries.

3.4.

3.4.

a

All phages were reduced using TCEP prior to cyclization. See reference for specific conditions.

b

Buffer A: 20 mM NH4CO3, 5 mM EDTA, pH 8.

c

Buffer B: 50 mM Tris pH 8.5.

d

Phages reduced with iTCEP for 48 h.

e

Buffer C: 55 μL of PBS (phage), 36 μL of water, 10 μL of 1 M sodium bicarbonate, pH 8.5.