FIG. 2.

GAL1-lacZ expression in yeast expressing REST/NRSF fragments. REST/NRSF repression of GAL1 transcription was quantified by replacing the HIS3 gene of pBM2389(RE1)₃UAS with lacZ. β-Galactosidase assays were performed as described in Materials and Methods. Results are the average of at least seven assays and standard errors were less than 10%. The percent activity is defined as the lacZ value with RE1/NRSEs divided by lacZ values without RE1/NRSEs multiplied by 100.