FIG. 5.

The N terminus of REST/NRSF recruits SIN3A and HDAC2 to the RE1/NRSE to mediate nucleosome deacetylation. Neuro-2A/1 cells were transfected with the plasmid pGL3LC.-677/+80 in combination with either pMTG4 (expressing the gal4 DNA binding domain) or pMTG4(1-138) (expressing gal4 DNA binding domain fused to the 138 N-terminal residues of REST/NRSF). Cells were cross-linked and immunoprecipitated with antibodies to acetylated histone H3, mSIN3A, HDAC2, or HA as described in Materials and Methods. Precipitated DNA was then PCR amplified with primers to the reporter plasmid around the M₄ RE1/NRSE and resolved on an agarose gel.