TABLE 1.
Stains and plasmids used in this study
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| S. cerevisiae strains FM242(YPH177) | MATα ura3-52 lys2-801 ade2-101 his3Δ200 trp1Δ63 leu2Δ1 cyh2 kar1Δ15 | 54 |
| yA1 | FM242(sin3::URA3) | This study |
| yA2 | FM242(sin3::URA3-HASIN3) | This study |
| yA3 | FM242(Δrpd3::HIS3) | This study |
| Plasmids | ||
| pBM2389 | TRP1 CEN GAL1TATA-HIS3 | 32a |
| pBM2389(RE1)3UAS | TRP1 CEN UASGAL1(RE1)3GAL1TATA-HIS3 | This study |
| pBM2389.LacZ | TRP1 CEN GAL1TATA-LacZ | This study |
| pAR1000 | TRP1 CEN UASGAL1(RE1)3GAL1TATA-LacZ | This study |
| pLEONOV | LEU2 2μm ADHpro/ADHterm | This study |