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. 2024 Apr 23;13(9):734. doi: 10.3390/cells13090734

Figure 1.

Figure 1

VPA functions as HDACi and REST/NRSF gene expression is up-regulated after CCI. (A) Immunoblot showing the pharmacologic effect of valproic acid (VPA) with increased acetyl-histone H3 levels in perilesional brain samples of VPA-treated mice (pooled from n = 11 per group) Antibodies used: 1: acetyl-histone H3 (Lys9) (C5B11); 2: acetyl-histone H3 (Lys27) (D5E4); 3: acetyl-histone H3 (Lys18) (D8Z5H); 4: acetyl-histone H3 (Lys14) (D4B9); 5: acetyl-histone H3 (Lys56); 6: histone H3 (D1H2). (B) Gene expression analyses and quantification of the transcription factor REST/NRSF normalized to Ppia in naïve animals (n = 10) and at 1 (n = 9 animals), 3, 5, and 7 (each time point n = 10 animals) days after CCI. Data are expressed as mean ± SEM with individual values shown and p values were calculated by one-way ANOVA with post hoc Holm–Šidák correction (*** p < 0.001; **** p < 0.0001).