Figure 1.

L-Glyceraldehyde inhibits cell growth in neuroblastoma cells. (A) Cell lines VH-7, IMR-32 and SH-SY5Y cells were incubated with varying concentrations of L-GA for 24 h after seeding. Viable cell counts were taken for each L-GA concentration via the trypan exclusion method and normalized to 0.0 mM control. The 50% reduction in cell count is denoted by a dotted red line with errors bar representing the ± SEM (n = 3). (B) Cell lines were incubated with 1 mM L-GA (blue) or PBS (black) for 48 hrs after seeding and settling for 24 h. Viable cell counts were taken every 24 h via the trypan exclusion method. Error bar representing the ± SE of the mean (n = 3). (C) Cell lines BE(2)-C, IMR5/75, SH-SY5Y, SK-N-AS, CLB-GA, GI-M-EN and VH-7 were treated with L-GA for 72 h before flow cytometry analysis. Sub-G1 cells were gated, and a two-sided T-test was performed (n = 3). ** $p\le$0.01; *** $p\le$0.001; **** $p\le$0.0001.