FIG. 3.

Regulation of GGPP synthase mRNA expression by insulin in 3T3-L1 adipocytes. Fully differentiated 3T3-L1 adipocytes were starved from serum for 24 h and then incubated in the absence (−) or presence (+) of 1 μM insulin for another 24 h. Poly(A) mRNA was purified from 150 μg of total RNA and analyzed by Northern blotting together with 10 μg of total RNA to show the relative level of expression of the different messages tested. The membrane was probed for GGPP synthase (top), stripped, and reprobed for fatty acid synthase as a positive control for insulin regulation of gene expression (middle) and for 36B4 as control for RNA loading (1) (bottom).