Figure 5.

TRIM44 promotes RABV replication by activating autophagy. NA cells were transfected with p-TRIM44 or siTRIM44 for 24 h, treated with 3-MA (5 mM) or rapamycin (0.1 μM), and then infected with RABV for 24 h at an MOI of 1. The relative expression levels of the RABV-G, TRIM44, and LC3II proteins were determined by Western blotting (A,B). The formation of LC3II-positive puncta was observed by laser confocal microscopy, Bar = 10 μm (C,D). The virus titers were evaluated using the TCID50 method (E,F). Statistical analysis was performed using one-way ANOVA. ns, p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.