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. 2024 Apr 23;16(9):1615. doi: 10.3390/cancers16091615

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Workflow of the Akoya PhenoCycler Fusion. FFPE tissue sections are stained with antibody panels linked to oligonucleotide barcodes. The PhenoCycler cycles through staining, imaging, and barcode removal, enabling visualization of a comprehensive set of markers. Following antibody incubation, fluorescent reporters bind to the complementary barcode on the antibody. Three reporters are added at a time, and subsequent isothermic washes allow the addition of the following set of reporters. Created with Biorender.com.