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. 2024 Apr 23;13(9):728. doi: 10.3390/cells13090728

Figure 7.

Figure 7

Effects of exogenous activin A on the infiltration of NK cells in NS-1 cell solid tumors. (A) The expression of activin-βA mRNA in NS-1 and 4T-1 cells was detected by RT-PCR (n = 3). ** p < 0.01, compared with the NS-l group (Student’s t test; mean ± S.D.). (B) The level of activin A protein in the culture supernatants of NS-1 and 4T-1 cells was determined by ELISA (n = 3). ** p < 0.01, compared with the NS-l group (Student’s t test; mean ± S.D.). (C–E) The NS-1 cell solid tumors were injected with 1 μL normal saline (Control) or 20 ng activin A in 1 μL normal saline every 24 h, three times continuously, and then (C) the body weight of mice was recorded, (D) tumor volume was measured, (E) and the percentage of NK cells infiltrating the tumor was examined by flow cytometry (n = 4). * p < 0.05, compared with the control group (n = 4) (Student’s t test; mean ± S.D.).