FIG. 1.

MSP prevents anoikis in RE7 cells. For induction of anoikis, epithelial cells from regular petri dishes were transferred to poly-Hema-coated dishes. Poly-Hema prevents the attachment of cells to plastic, and nonattached cells undergo apoptosis, which was quantified by TUNEL staining after overnight incubation in serum-free medium (A and B) or in the presence of 5 nM MSP (C and D). Cells attached to uncoated petri dishes in serum-free medium were also evaluated (E and F). Left panels, 4′,6-diamidino-2-phenylindole (DAPI)-stained blue nuclei demonstrating the total number of cells in the field; right panels, fluorescein isothiocyanate-stained apoptotic cells in the same field.