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Adhesion promotes translocation of MSP-activated MAPK from the cytoplasm into the nucleus. Starved RE7 epithelial cells were stimulated with 5 nM MSP for 15 min in suspension or on coverslips, and then translocation of MAPK was detected by indirect immunofluorescence analysis (A) or by Western blotting of cytoplasmic and nuclear cell extracts (B) with antibodies to phosphoMAPK (pMAPK) (top) or total MAPK (bottom).
